Cloned (Comment) | Organism |
---|---|
cDNA cloned and expressed in the methylotrophic yeast Pichia pastoris as protryptase | Homo sapiens |
General Stability | Organism |
---|---|
stabilized by heparin proteoglycans | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
APC-1167 | - |
Homo sapiens | |
APC-366 | - |
Homo sapiens | |
Aprotinin | weak inhibitor | Homo sapiens | |
BABIM | - |
Homo sapiens | |
additional information | no inhibition of native or recombinant enzyme by soybean trypsin inhibitor SBTI | Homo sapiens | |
Pentamidine | nonselective inhibitor | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.5 | - |
N-p-tosyl-Gly-Pro-Lys-p-nitroanilide | pH 8.2, 22°C, recombinant tryptase | Homo sapiens | |
4.2 | - |
N-p-tosyl-Gly-Pro-Lys-p-nitroanilide | pH 8.2, 22°C, HMC-1 tryptase | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
secretory granule | - |
Homo sapiens | 30141 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
human | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
fibrinogen + H2O | - |
Homo sapiens | fibrinogen fragments | - |
? | |
N-p-tosyl-Gly-Pro-Lys-p-nitroanilide + H2O | synthetic peptide substrate | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
HMC-1 tryptase | - |
Homo sapiens |
Mast cell tryptase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
490 | - |
N-p-tosyl-Gly-Pro-Lys-p-nitroanilide | pH 8.2, 22°C, recombinant tryptase | Homo sapiens | |
540 | - |
N-p-tosyl-Gly-Pro-Lys-p-nitroanilide | pH 8.2, 22°C, HMC-1 tryptase | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
APC-366 | pH 8.2, 22°C, recombinant tryptase | Homo sapiens | |
0.0023 | - |
Pentamidine | pH 8.2, 22°C, recombinant tryptase | Homo sapiens | |
0.0025 | - |
BABIM | pH 8.2, 22°C, recombinant tryptase | Homo sapiens | |
0.0028 | - |
Pentamidine | pH 8.2, 22°C | Homo sapiens | |
0.0028 | - |
APC-366 | pH 8.2, 22°C | Homo sapiens | |
0.0046 | - |
BABIM | pH 8.2, 22°C | Homo sapiens | |
0.0092 | - |
APC-1167 | pH 8.2, 22°C, recombinant tryptase | Homo sapiens | |
0.0097 | - |
APC-1167 | pH 8.2, 22°C | Homo sapiens | |
0.25 | - |
Aprotinin | pH 8.2, 22°C | Homo sapiens | |
0.369 | - |
Aprotinin | pH 8.2, 22°C, recombinant tryptase | Homo sapiens |